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goat anti ahr antibody  (Novus Biologicals)


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    Novus Biologicals goat anti ahr antibody
    Figure 1. Endogenous <t>AHR</t> and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with <t>AHR,</t> <t>ARNT</t> and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.
    Goat Anti Ahr Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+ahr+antibody/AHR+Antibody/pm39207053-312-15-19
    Average 93 stars, based on 12 article reviews
    goat anti ahr antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear Translocation and Target Gene Activation."

    Article Title: Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear Translocation and Target Gene Activation.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    doi: 10.1002/advs.202400794

    Figure 1. Endogenous AHR and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with AHR, ARNT and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.
    Figure Legend Snippet: Figure 1. Endogenous AHR and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with AHR, ARNT and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.

    Techniques Used: Fractionation, Immunoprecipitation, Western Blot, Control, Double Staining

    Figure 8. Schematic illustration of the dual function of SPHK2. A) SPHK2 functions as a cofactor for the AHR/ARNT heterodimer on the DRE-containing promoter region of the CYP1A1 gene. B) SPHK2, S1P, and AHR establish a positive feedback mechanism for ceramide de novo biosynthesis metabolism. S1P also enhances AHR recruitment to DREs.
    Figure Legend Snippet: Figure 8. Schematic illustration of the dual function of SPHK2. A) SPHK2 functions as a cofactor for the AHR/ARNT heterodimer on the DRE-containing promoter region of the CYP1A1 gene. B) SPHK2, S1P, and AHR establish a positive feedback mechanism for ceramide de novo biosynthesis metabolism. S1P also enhances AHR recruitment to DREs.

    Techniques Used:

    Related Articles

    Western Blot:

    Article Title: Genistein Promotes M2 Macrophage Polarization via Aryl Hydrocarbon Receptor and Alleviates Intestinal Inflammation in Broilers with Necrotic Enteritis
    Article Snippet: Trizol reagent, PrimeScriptTM RT Reagent Kit with gDNA Eraser, and TB Green ® Premix Ex Taq TM II were from Takara (Kusatsu, Japan). .. Antibodies used in Western blot and immunofluorescence included the following: rabbit anti-AhR (MBS829549, Mybiosoure, San Diego, CA, USA), goat anti-AhR antibody (NB100-128, Novus, Littleton, CO, USA), rabbit anti-CYP1A1 (D120518, Sangon biotech, Shanghai, China), rabbit anti-CD163 antibody (A8383, ABclonal, Wuhan, China), rabbit anti-Arginase-1(Arg-1, GB11285, Service-bio, Wuhan, China), mouse anti-β-actin (30101ES10, Yeasen, Shanghai, China), mouse anti-β-tubulin (M20005, Abmart, Shanghai, China), Alexa FluorTM 488-conjugated anti-goat (A11055, ThermoFisher) and Alexa Fluor 647-conjugated anti-rabbit secondary antibody (A0468, Beyotime, Shanghai, China). ..

    Immunofluorescence:

    Article Title: Genistein Promotes M2 Macrophage Polarization via Aryl Hydrocarbon Receptor and Alleviates Intestinal Inflammation in Broilers with Necrotic Enteritis
    Article Snippet: Trizol reagent, PrimeScriptTM RT Reagent Kit with gDNA Eraser, and TB Green ® Premix Ex Taq TM II were from Takara (Kusatsu, Japan). .. Antibodies used in Western blot and immunofluorescence included the following: rabbit anti-AhR (MBS829549, Mybiosoure, San Diego, CA, USA), goat anti-AhR antibody (NB100-128, Novus, Littleton, CO, USA), rabbit anti-CYP1A1 (D120518, Sangon biotech, Shanghai, China), rabbit anti-CD163 antibody (A8383, ABclonal, Wuhan, China), rabbit anti-Arginase-1(Arg-1, GB11285, Service-bio, Wuhan, China), mouse anti-β-actin (30101ES10, Yeasen, Shanghai, China), mouse anti-β-tubulin (M20005, Abmart, Shanghai, China), Alexa FluorTM 488-conjugated anti-goat (A11055, ThermoFisher) and Alexa Fluor 647-conjugated anti-rabbit secondary antibody (A0468, Beyotime, Shanghai, China). ..



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    Figure 1. Endogenous <t>AHR</t> and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with <t>AHR,</t> <t>ARNT</t> and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.
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    Image Search Results


    Figure 1. Endogenous AHR and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with AHR, ARNT and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear Translocation and Target Gene Activation.

    doi: 10.1002/advs.202400794

    Figure Lengend Snippet: Figure 1. Endogenous AHR and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with AHR, ARNT and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti-AHR and anti-SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 μm. Data are representative images from three independent experiments.

    Article Snippet: Antibodies: Primary antibodies used were as follows: AHR: AHR Rabbit mAb (D5S6H, Cell Signaling technology), Goat anti-AHR antibody (#NB100-128, Novus Biologicals), Mouse monoclonal Anti-AHR #14- 9854-82, Invitrogen), ARNT: HIF-1b/ARNT Rabbit mAb (D28F3, Cell Signaling Technology), SPHK2: SPHK2 Rabbit mAb (D2V3G, Cell Signaling Technology), Phospho-SPHK2: Anti-Phospho-SphK2 (T614) Antibody (#A01382T614, Bosterbio), SPTLC1: anti-SPTLC1 antibody (ab176706, abcam), SPTLC2: anti-Serine Palmitoyltransferase antibody (ab236900, abcam), SPHK1: SPHK1 (D1H1L) Rabbit mAb (#12 071, Cell Signaling Technology), S1P1: S1P1 Polyclonal Antibody (#PA1-1040, Thermo Scientific), Actinb: β-Actin (13E5) Rabbit mAb (#4970, Cell signaling Technology), GAPDH: GAPDH (D16H11) XP Rabbit mAb (#5174, Cell Signaling Technology), Histon H3: Histone H3 (D1H2) XP Rabbit mAb (#4499, Cell Signaling Technology), HA: HA-Tag Rabbit mAb (C29F4, Cell Signaling Technology).

    Techniques: Fractionation, Immunoprecipitation, Western Blot, Control, Double Staining

    Figure 8. Schematic illustration of the dual function of SPHK2. A) SPHK2 functions as a cofactor for the AHR/ARNT heterodimer on the DRE-containing promoter region of the CYP1A1 gene. B) SPHK2, S1P, and AHR establish a positive feedback mechanism for ceramide de novo biosynthesis metabolism. S1P also enhances AHR recruitment to DREs.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear Translocation and Target Gene Activation.

    doi: 10.1002/advs.202400794

    Figure Lengend Snippet: Figure 8. Schematic illustration of the dual function of SPHK2. A) SPHK2 functions as a cofactor for the AHR/ARNT heterodimer on the DRE-containing promoter region of the CYP1A1 gene. B) SPHK2, S1P, and AHR establish a positive feedback mechanism for ceramide de novo biosynthesis metabolism. S1P also enhances AHR recruitment to DREs.

    Article Snippet: Antibodies: Primary antibodies used were as follows: AHR: AHR Rabbit mAb (D5S6H, Cell Signaling technology), Goat anti-AHR antibody (#NB100-128, Novus Biologicals), Mouse monoclonal Anti-AHR #14- 9854-82, Invitrogen), ARNT: HIF-1b/ARNT Rabbit mAb (D28F3, Cell Signaling Technology), SPHK2: SPHK2 Rabbit mAb (D2V3G, Cell Signaling Technology), Phospho-SPHK2: Anti-Phospho-SphK2 (T614) Antibody (#A01382T614, Bosterbio), SPTLC1: anti-SPTLC1 antibody (ab176706, abcam), SPTLC2: anti-Serine Palmitoyltransferase antibody (ab236900, abcam), SPHK1: SPHK1 (D1H1L) Rabbit mAb (#12 071, Cell Signaling Technology), S1P1: S1P1 Polyclonal Antibody (#PA1-1040, Thermo Scientific), Actinb: β-Actin (13E5) Rabbit mAb (#4970, Cell signaling Technology), GAPDH: GAPDH (D16H11) XP Rabbit mAb (#5174, Cell Signaling Technology), Histon H3: Histone H3 (D1H2) XP Rabbit mAb (#4499, Cell Signaling Technology), HA: HA-Tag Rabbit mAb (C29F4, Cell Signaling Technology).

    Techniques: